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. 2018 Aug 6;217(8):2743–2763. doi: 10.1083/jcb.201710116

Table 1. Strains used in this study.

Name Genotype Origin
BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Brachmann et al. (1998)
BY4742 MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 Brachmann et al. (1998)
BY4747 MATα his3Δ200 leu2Δ0 lys2Δ0 met15Δ0 trp1Δ63 ura3Δ0 Brachmann et al. (1998)
CUY4813 BY4741 hphNT1::GAL1pr-ATG2-mGFP-TAP::kanMX This study
CUY9058 BY4741xB4727 URA3::GAL1pr-ATG18-TAP::kanMX hphNT1::GAL1pr-ATG2 This study
CUY10110 BY4727 NatMX6::prGAL1-ATG9-3xFLAG::hphNT1 This study
CUY10771 BY4741 hphNT1::GAL1pr-ATG2 GAL1pr-ATG18FAAG-TAP::URA3 This study
CUY10811 BY4727 atg2Δ::hphNT1 URA3::GAL1pr-ATG18-TAP::kanMX cloNAT::GAL1pr-ATG2PM1::TRP1 This study
CUY10813 BY4727 atg2Δ::hphNT1 GAL1pr-ATG18FAAG-TAP::URA3 cloNAT::GAL1pr-ATG2PM1::TRP1 This study
CUY10934 SEY6210 ATG9-GFP::hphNT1 natNT2::ADH1pr-mCherry-ATG8 atg2Δ::HIS5S.p. This study
CUY10935 SEY6210 SEC63-GFP::kanMX natNT2::ADH1pr-mCherry-ATG8 atg2Δ::HIS5S.p. This study
CUY11160 BY4741 hphNT1::GAL1pr-ATG2-mGFP-TAP::kanMX atg18Δ::NatNT2 This study
CUY11161 BY4727 CloNAT::GAL1pr-ATG2PM1-TAP::kanMX-TRP1 atg2Δ::hphNT1 This study
ERY087 SEY6210 ATG2-GFP::HIS5S.p. atg18Δ::TRP1 Rieter et al. (2012)
FRY375 SEY6210 atg2Δ::HIS5S.p. This study
FRY382 PJ69-4A atg18Δ::TRP1 This study
FRY383 SEY6210 atg2Δ::LEU2 This study
FRY388 BY4742 pho13Δ::kanMX pho8::PHO8Δ60 atg2Δ::HIS5S.p. This study
KTY97 SEY6210 ATG9-GFP::TRP1 Reggiori et al. (2004)
PJ69-4A MATa leu2-3,112 trp1-Δ901 ura3-52 his3-Δ200 galΔ4 gal80Δ LYS::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ James et al. (1996)
RGY296 SEY6210 atg8Δ::kanMX atg2Δ::hphNT1 SEC63-GFP::NatMX6 pCumCheV5ATG8::URA3 pRS::TRP1 This study
RGY303 SEY6210 atg8Δ::kanMX atg2Δ::hphNT1SEC63-GFP::NatMX6 pCumCheV5ATG8::URA3 pATG2::TRP1 This study
RGY304 SEY6210 atg8Δ::kanMX atg2Δ::hphNT1 SEC63-GFP::NatMX6 pCumCheV5ATG8::URA3 pATG2PM1::TRP1 This study
RGY305 SEY6210 atg8Δ::kanMX atg2Δ::hphNT1 SEC63-GFP::NatMX6 pCumCheV5ATG8::URA3 pATG2PM4::TRP1 This study
RGY528 SEY6210 atg8Δ::LoxP-kanMX-LoxP pCumCheV5ATG8::URA3 ATG18-GFP::NatMX6 atg2Δ::hphNT1 pRS405::LEU2 This study
RGY529 SEY6210 atg8Δ::LoxP-kanMX-LoxP pCumCheV5ATG8::URA3 ATG18-GFP::NatMX6 atg2Δ::hphNT1 pATG2-TAP::LEU2 This study
RGY530 SEY6210 atg8Δ::LoxP-kanMX-LoxP pCumCheV5ATG8::URA3 ATG18-GFP::NatMX6 atg2Δ::hphNT1 pATG2PM1-TAP::LEU2 This study
RGY531 SEY6210 atg8Δ::LoxP-kanMX-LoxP pCumCheV5ATG8::URA3 ATG18-GFP::NatMX6 atg2Δ::hphNT1 pATG2PM4-TAP::LEU2 This study
RGY562 SEY6210 ATG18 × 13myc::TRP1 atg2Δ::HIS5S.p. pATG2-TAP::LEU2 atg9Δ::URA3K.l. This study
RHY030 SEY6210 ATG9-VN::HIS3 atg2Δ::TRP1 pCumCheV5ATG8::URA3 This study
RHY031 SEY6210 ATG9-VN::HIS3 atg2Δ::TRP1 pCumCheV5ATG8::URA3 pATG2-VC::LEU2 This study
RHY032 SEY6210 ATG9-VN::HIS3 atg2Δ::TRP1 pCumCheV5ATG8::URA3 pATG2PM1-VC::LEU2 This study
RHY033 SEY6210 ATG9-VN::HIS3 atg2Δ::TRP1 pCumCheV5ATG8::URA3 pATG2PM4-VC::LEU2 This study
RHY034 SEY6210 SEC63-GFP::kanMX natNT1:: ADH1pr-mCherry-ATG8 atg2Δ::HIS5S.p. atg9Δ::TRP1 This study
RHY038 SEY6210 ATG1-VC::TRP1 atg2Δ::hphMX6 promATG2-VN::LEU2 pCumCheV5ATG8::URA3 This study
RHY040 SEY6210 ATG2-VN::HIS5S.p. pCumCheV5ATG8::URA3 promATG1-VC::LEU2 This study
RSGY003 SEY6210 ATG9-GFP::TRP1 atg2Δ::HIS5S.p. pATG2-TAP::LEU2 This study
RSGY004 SEY6210 ATG9-GFP::TRP1 atg2Δ::HIS5S.p. pATG2PM1-TAP::LEU2 This study
RSGY005 SEY6210 ATG9-GFP::TRP1 atg2Δ::HIS5S.p. pATG2PM4-TAP::LEU2 This study
RSGY011 SEY6210 ATG18 × 13myc::TRP1 atg2Δ::HIS5S.p. This study
RSGY012 SEY6210 ATG18 × 13myc::TRP1 atg2Δ::HIS5S.p. pATG2-TAP::LEU2 This study
RSGY013 SEY6210 ATG18 × 13myc::TRP1 atg2Δ::HIS5S.p. pATG2PM1-TAP::LEU2 This study
RSGY014 SEY6210 ATG18 × 13myc::TRP1 atg2Δ::HIS5S.p. pATG2PM4-TAP::LEU2 This study
RSGY015 SEY6210 ATG18 × 13myc::TRP1 atg2Δ::HIS5S.p. pRS::LEU2 This study
RSGY017 SEY6210 ATG18-GFP::TRP1 pCumCheV5ATG8::URA3 This study
RSGY018 SEY6210 ATG18-GFP::TRP1 pCumCheV5ATG8::URA3 atg2Δ::HIS5S.p. This study
RSGY019 SEY6210 ATG18-GFP::TRP1 pCumCheV5ATG8::URA3 atg2Δ::HIS5S.p. pATG2-TAP::LEU2 This study
RSGY020 SEY6210 ATG18-GFP::TRP1 pCumCheV5ATG8::URA3 atg2Δ::HIS S.p. pATG2PM1-TAP::LEU2 This study
RSGY021 SEY6210 ATG18-GFP::TRP1 pCumCheV5ATG8::URA3 .atg2Δ::HIS5S.p pATG2PM4-TAP::LEU2 This study
RSGY024 SEY6210 atg2Δ::HIS5S.p. pRS::LEU2 pCumCheV5ATG8::URA3 This study
RSGY025 SEY6210 atg2Δ::HIS5S.p. pATG2PM1-TAP::LEU2 pCumCheV5ATG8::URA3 This study
RSGY026 SEY6210 atg2Δ::HIS5S.p. pATG2PM4-TAP::LEU2 pCumCheV5ATG8::URA3 This study
RSGY040 W303 pCumCheV5Atg8::URA3 atg2Δ::TRP1 This study
RSGY041 W303 pCumCheV5Atg8::URA3 atg2Δ::HIS5S.p. pATG2-TAP::LEU2 This study
RSGY042 W303 pCumCheV5Atg8::URA3 atg2Δ::HIS5S.p. pATG2PM1-TAP::LEU2 This study
RSGY043 W303 pCumCheV5Atg8::URA3 atg2Δ::HIS5S.p. pATG2PM4-TAP::LEU2 This study
RSGY052 W303 atg2Δ::TRP1 pATG2-TAP::LEU2 This study
RSGY053 W303 atg2Δ::TRP1 This study
RSGY054 W303 atg2Δ::TRP1 pATG2PM1-TAP::LEU2 This study
RSGY055 W303 atg2Δ::TRP1 pATG2PM4-TAP::LEU2 This study
RSGY087 SEY6210 ATG1-VC::TRP1 atg2Δ::hphMX6 pATG2-VN::LEU2pCumCheV5ATG8::URA3 This study
RSGY088 SEY6210 ATG1-VC::TRP1 atg2Δ::hphMX6 pCumCheV5ATG8::URA3 This study
RSGY089 SEY6210 ATG1-VC::TRP1 atg2Δ::hphMX6 pATG2PM1-VN::LEU2pCumCheV5ATG8::URA3 This study
RSGY090 SEY6210 ATG1-VC::TRP1 atg2Δ::hphMX6 pATG2PM4-VN::LEU2pCumCheV5ATG8::URA3 This study
SAY118 SEY6210 ATG9-GFP::TRP1 atg2Δ::HIS5S.p. This study
SEY6210 MATα ura3-52 leu2-3,112 his3-Δ200 trp1-Δ901 lys2-801suc2-Δ9 mel GAL Robinson et al. (1988)
VDY101 SEY6210 atg7Δ::LEU2 Lab stock
W303 MATα ade2–1 leu2–3 his3–11, 15 trp1–1 ura3–1 can1–100 Graef et al. (2013)
yCK759 BY4742 atg2Δ::kanMX This study
yDP29 BY4741 atg2Δ:: hphNT1 atg9Δ::kanMX This study
yDP191 BY4741 MET15 atg2Δ::kanMX ATG9-GFP::URA3 This study
yDP264 BY4741 MET15 atg2Δ::kanMX atg18Δ::kanMX This study