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. 2018 Aug 6;217(8):2675–2690. doi: 10.1083/jcb.201706164

Figure 1.

Figure 1.

TORC1 inactivation triggers intranuclear repositioning of nucleolar proteins and rDNA. (A) Exponentially growing cells of strain SCU3287 (NVJ1-GFP) harboring plasmid pSCU618 (pRFP-NOP1) were treated with 200 ng/ml rapamycin for 1 h. Cell images with GFP and RFP signals were captured using a fluorescence microscope. Bars, 2.5 µm. The distance between Nvj1-GFP and RFP-Nop1 peaks, measured as described in Materials and methods (also see Fig. S1 A), is shown in the box plot. Numbers above the bars are sample sizes. P-values were calculated using a two-tailed Mann–Whitney U test. (B) Cells of strain SCU4433 (NVJ1-GFP NET1-mRuby2) were treated with rapamycin for 1 h. Bars, 2.5 µm. The distance between the Nvj1-GFP and Net1-RFP peaks is shown in the box plot. (C) Cells of strain SCU359 (FOB1-GFP) harboring plasmid pSCU618 (pRFP-NOP1) were treated with rapamycin for 1 h. Bars, 2.5 µm. The distance between Fob1-GFP and RFP-Nop1 peaks, measured as described in Materials and methods (also see Fig. S1 C), is shown in the box plot. (D) Cells of strain SCU4449 (NET1-mRuby2) harboring plasmid pSCU740 (pNOP1-GFP) were treated with rapamycin for 1 h. Bars, 2.5 µm. The distance between the Nop1-GFP and Net1-RFP peaks is shown in the box plot.