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. 2018 Aug 6;217(8):2799–2812. doi: 10.1083/jcb.201710022

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© 2018 Wang et al.

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Figure 2. — Integrin α4β7 conformational changes induced by chemokines and Mn²⁺. (A) Experiment setup for measuring FRET efficiency between integrin α4β7 βI domain and the plasma membrane (ectodomain FRET) and FRET efficiency between integrin α4 and β7 cytoplasmic domains (cytoplasmic domain FRET). A composite of all molecules used is depicted. (B and C) Ectodomain FRET efficiency (B) and cytoplasmic domain FRET efficiency (C) in cells before and after treatment with indicated concentration of chemokines or 0.5 mM Mn²⁺. (D and E) Ectodomain FRET efficiency (D) and cytoplasmic domain FRET efficiency (E) in cells over a 30-min time course of treatment with 0.5 µg/ml chemokines or 0.5 mM Mn²⁺. Data are represented as mean ± SEM of 6–12 cells per condition (n = 6–12). Two-tailed Student’s t test. **, P < 0.01; ***, P < 0.001.