Figure 1.

KIF3B is an essential component of the kinesin-II trimer. (A and B) Lysates prepared from HEK293T cells coexpressing the indicated combination of EGFP-fused and mChe-fused kinesin-II subunits were processed for the VIP assay using GST–anti-GFP Nb prebound to glutathione–Sepharose beads. EGFP (A) and mChe (B) signals on the precipitated beads were observed under a microscope. Bars, 100 µm. (C) Kinesin-II models predicted from the binary interaction data. (D–I) Control RPE1 cells (D and G) and KIF3B-KO cell lines 3B-2-1 (E and H) and 3B-2-4 (F and I) were serum-starved to induce ciliogenesis and triple immunostained for either IFT88 (D–F) or IFT140 (G–I), together with Ac-α-tubulin (D′–I′) and γ-tubulin (D″–I″). Bars: (main images) 10 µm; (insets) 5 µm.