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. 2018 Aug 6;217(8):2867–2876. doi: 10.1083/jcb.201801039

Figure 5.

Figure 5.

KIF3B–IFT-B interaction is essential for ciliogenesis. (A–F) The KIF3B-KO cell line 3B-2-1 stably expressing EGFP-fused KIF3B(WT) (A), KIF3B(1–704) (B), KIF3B(1–662) (C), KIF3B(1–625) (D), KIF3B(1–579) (E), or EGFP (F) were triple immunostained for Ac-α-tubulin (A–F), γ-tubulin (A′–F′), and GFP (A″–F″). Bars: (main images) 10 µm; (insets) 5 µm. (G) Ciliated cells of the KIF3B-KO cell lines (3B-2-1 and 3B-2-4) stably expressing EGFP-fused KIF3B(WT), KIF3B(1–704), or KIF3B(1–662) were counted, and percentages of ciliated cells are represented as bar graphs. Values are shown as means ± SEM of three independent experiments. In each set of experiments, 31–50 cells with EGFP signals were analyzed, and the total number of cells analyzed (n) for each sample is shown.