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. 2018 Aug 6;217(8):2691–2708. doi: 10.1083/jcb.201802138

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© 2018 McIntosh et al.

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Figure 6. — Shapes of plus ends of in vitro MTs elongating or prepared under stabilizing conditions. (A) Tomographic slices and models of MT ends plunge frozen while elongating in GMPCPP. (B) Tomographic slices and models of MTs fixed isothermally in first 0.2% and then 2% glutaraldehyde before plunge freezing. (C) Tomographic slices and models of MTs fixed isothermally in first 0.2% and then 2% glutaraldehyde and then negatively strained with uranyl acetate. Red crosses mark the origins of the coordinate systems used. Bar, 50 nm.