Table 3. Properties of PFs on MTs and of tubulin in vitro.
Dataset | PF length | PF curvature | # PFs |
---|---|---|---|
nm | Deg/dimer | ||
GTP | 36 ± 15 | 19.3 ± 13.1 | 884 |
GMPCPP | 38 ± 19 | 18.6 ± 12.8 | 271 |
Fixed-plunge | 25 ± 13 | 19.9 ± 13.6 | 264 |
Fixed-NegStain | 21 ± 10 | 20.5 ± 13.3 | 365 |
Depoly | 43 ± 16 | 20.2 ± 12.1 | 365 |
Extrapolated tip curvature: GTP growth | 23.6 ± 0.8 | ||
Extrapolated tip curvature: GMPCPP | 22.3 ± 0.7 | ||
Extrapolated tip curvature: MT shorten | 24.1 ± 0.6 | ||
GTP-tubulin–darpin complex | 22 | PDB ID 4DRX | |
GMPCPP-tubulin–stathmin complex | 19 | PDB ID 3RYH | |
GDP-tubulin–colchicine-stathmin | 26 | PDB ID 1SA0 |