Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (E.coli) | BL21 Star (DE3) | ThermoFisher Scientific | C601003 | Chemically Competent E.coli for the expression of recombinant RTs. |
| Strain, strain background (HIV-1 IIIB) | HIV-1 L100I | Established in house | ||
| Strain, strain background (HIV-1 IIIB) | HIV-1 K103N | Established in house | ||
| Strain, strain background (HIV-1 IIIB) | HIV-1 E138K | Established in house | ||
| Strain, strain background (HIV-1 IIIB) | HIV-1 Y181C | Established in house | ||
| Strain, strain background (HIV-1 IIIB) | HIV-1 K103N/Y181C | Established in house | ||
| Cell line (H. Sapiens) | MT-4 cells | NIH AIDS Reagent Program |
NIH-ARP Cat# 120–438, RRID:CVCL_2632 |
|
| Recombinant DNA reagent | pCDFDuet-1 | Millipore Sigma | 71340–3 | Expression plasmid for all RT variants in E.coli. |
| Peptide, recombinant protein | HRV 3C protease | Recombinantly expressed in house |
Expressed as His-tagged fusion protein. |
|
| Commercial assay or kit | EnzChek Reverse Transcriptase Assay kit |
ThermoFisher Scientific | E22064 | |
| Chemical compound, drug | Compound K-5a2 | Synthesized in house | ||
| Chemical compound, drug | Compound 25a | Synthesized in house | ||
| Chemical compound, drug | etravirine | Sigma-Aldrich | ADV428293567 | |
| Chemical compound, drug | rilpivirine | Sigma-Aldrich | ADV465749297 | |
| Chemical compound, drug | PicoGreen dsDNA reagent |
ThermoFisher Scientific | P7581 | |
| Software, algorithm | Coot | (Emsley et al., 2010) | RRID:SCR_014222 | |
| Software, algorithm | XDS | (Kabsch, 2010) | RRID:SCR_015652 | |
| Software, algorithm | PHASER | (McCoy et al., 2007) | RRID:SCR_014219 | |
| Software, algorithm | PHENIX suite | (Adams et al., 2010) | RRID:SCR_014224 | |
| Software, algorithm | PyMol v 2.0 | The PyMOL Molecular Graphics System, Schrödinger, LLC. |
RRID:SCR_000305 | |
| Software, algorithm | MacroModel | Schrödinger suite | RRID:SCR_014879 | |
| Software, algorithm | UCSF Chimera | (Pettersen et al., 2004) | RRID:SCR_004097 | |
| Software, algorithm | UCSF ChimeraX | (Goddard et al., 2018) | RRID:SCR_015872 | |
| Software, algorithm | GraphPad Prism v 7.0a | GraphPad Software | RRID:SCR_002798 |