Table 2. In vitro inhibition of HIV-1 reverse transcriptase by 25a and RPV.
| RT variants | 25a | RPV | ||||
|---|---|---|---|---|---|---|
| IC50 (nM) | Fold R* | Curve slope | IC50 (nM) | Fold R | Curve slope | |
| WT | 4.3 ± 0.080† | – | 3.8 ± 0.70 | 3.5 ± 0.052 | – | 3.1 ± 0.17 |
| K103N/Y181C | 31 ± 0.83 | 7.2 | 1.9 ± 0.092 | 51 ± 1.5 | 15 | 1.4 ± 0.047 |
| Y188L | 3.0 ± 0.15 | 0.70 | 1.9 ± 0.16 | 7.6 ± 0.22 | 2.2 | 1.7 ± 0.069 |
| V106A/F227L | 7.3 ± 0.23 | 1.7 | 3.1 ± 0.17 | 14 ± 0.27 | 4.0 | 2.1 ± 0.083 |
| K101P | 5.4 ± 0.16 | 1.3 | 2.9 ± 0.21 | 71 ± 3.0 | 20 | 1.2 ± 0.056 |
| Y181I | 38 ± 1.1 | 8.8 | 2.3 ± 0.16 | 315 ± 15 | 90 | 1.4 ± 0.081 |
| K103N/Y181I | 412 ± 13 | 96 | 1.8 ± 0.094 | 6317 ± 339 | 1805 | 1.4 ± 0.10 |
| P225H | 2.5 ± 0.059 | 0.58 | 3.5 ± 0.15 | 3.7 ± 0.070 | 1.1 | 3.4 ± 0.34 |
| P236L | 2.6 ± 0.13 | 0.60 | 4.3 ± 0.43 | 3.7 ± 0.085 | 1.1 | 3.6 ± 0.47 |
* Mean fold change in the IC50 values of mutant RT versus WT RT.
† Data reported as mean ±standard error.