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. 2018 Aug 7;7:e36745. doi: 10.7554/eLife.36745

Figure 6. Num1 localization at the bud tip in scs2/22∆ requires Bni1.

(A) Wide-field images of Num1-GFP in WT, scs2/22∆, bni1∆, and bni1∆ scs2/22∆ cells. Each image is a maximum intensity projection of 7 optical sections spaced 0.5 µm apart. (B) Percentage of cells with Num1-GFP patch at the bud tip is decreased in bni1∆ scs2/22∆ mutant relative to scs2/22∆ mutant (n ≥ 81 cells per strain). Error bars indicate SEP. ****p<0.0001 by unpaired t test. (C) Percentage of misaligned anaphase spindle for WT, scs2/22∆, bni1∆, and bni1∆ scs2/22∆ cells (n ≥ 83 per strain). Error bars indicate SEP. ***p<0.0001 by one-way ANOVA test. (D) Num1-GFP localization in WT and scs2/22∆ cells treated with DMSO or 200 µM latrunculin A for 20 min.

Figure 6.

Figure 6—figure supplement 1. Control experiment showing F-actin disassembly by latrunculin A.

Figure 6—figure supplement 1.

Rhodamine-phalloidin staining of WT cells treated with DMSO or 200 µM latrunculin A for 10 min (top row) and 20 min (bottom row).