Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-c-Myc (mouse monoclonal) |
BioLegend | BioLegend:626802; RRID:AB_2148451 |
(1:250 or 500 or 1000) |
| Antibody | Rabbit IgG | GenScript | GenScript:A01008; RRID:AB_2732863 |
(1:5000) |
| Antibody | HRP goat anti-mouse IgG | BioLegend | BioLegend:405306; RRID:AB_315009 |
(1:10000) |
| Antibody | HRP goat anti-rabbit IgG | Jackson Immuno Research Labs |
Jackson ImmunoResearch Labs: 111-035-144; RRID:AB_2307391 |
(1:10000) |
| Antibody | anti-Sac1 (rabbit polyclonal) |
Charles Barlowe Lab | NA | (1:2000) |
| Gene (Saccharomyces cerevisiae) |
NUM1 | NA | SGD:S000002557 | Systematic name: YDR150W |
| Gene (S. cerevisiae) | SCS2 | NA | SGD:S000000922 | Systematic name: YER120W |
| Gene (S. cerevisiae) | SCS22 | NA | SGD:S000007228 | Systematic name: YBL091C-A |
| Gene (S. cerevisiae) | DYN1 | NA | SGD:S000001762 | Systematic name: YKR054C |
| Gene (S. cerevisiae) | NIP100 | NA | SGD:S000006095 | Systematic name: YPL174C |
| Gene (S. cerevisiae) | JNM1 | NA | SGD:S000004908 | Systematic name: YMR294W |
| Gene (S. cerevisiae) | ARP1 | NA | SGD:S000001171 | Systematic name: YHR129C |
| Gene (S. cerevisiae) | KAR9 | NA | SGD:S000006190 | Systematic name: YPL269W |
| Gene (S. cerevisiae) | BNI1 | NA | SGD:S000005215 | Systematic name: YNL271C |
| Gene (S. cerevisiae) | MMR1 | NA | SGD:S000004180 | Systematic name: YLR190W |
| Gene (S. cerevisiae) | GEM1 | NA | SGD:S000000046 | Systematic name: YAL048C |
| Gene (S. cerevisiae) | PAC1 | NA | SGD:S000005795 | Systematic name: YOR269W |
| Gene (S. cerevisiae) | CIN8 | NA | SGD:S000000787 | Systematic name: YEL061C |
| Gene (S. cerevisiae) | KIP3 | NA | SGD:S000003184 | Systematic name: YGL216W |
| Gene (S. cerevisiae) | KAR3 | NA | SGD:S000006345 | Systematic name: YPR141C |
| Chemical compound, drug |
Hydroxyurea | Thermo Fisher Scientific |
Thermo Fisher Scientific: AC151680250 |
(200 mM) |
| Chemical compound, drug |
Latrunculin A | Millipore Sigma | Millipore Sigma:L5163 | (200 µM) |
| Chemical compound, drug |
Hygromycin B | Gold Biotechnology | Gold Biotechnology: H-270–5 |
(400 µg/ml) |
| Chemical compound, drug |
G-418 sulfate | Gold Biotechnology | Gold Biotechnology: G-418–10 |
(400 µg/ml) |
| Software, algorithm | NIS-Elements | Nikon | RRID:SCR_014329 | |
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
| Software, algorithm | Prism 7 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | Illustrator | Adobe | RRID:SCR_010279 | |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | |
| Other | Rhodamine-phalloidin | Cytoskeleton | Cytoskeleton:PHDR1 | (1.4 µM) |
| Other | Protease inhibitor cocktail tablet |
Millipore Sigma | Millipore Sigma:5892970001 | (1 tablet per 7 ml) |