Table 1. Protein targets that are covalently modified by 147.
Table 1—source data 1. Excel spreadsheet showing the competition ratio data for all 90 proteins that were identified as targets of 147 in every replicate and every cell type.
elife-37168-table1-data1.xlsx (22.3KB, xlsx)
DOI: 10.7554/eLife.37168.009
Table 1—source data 2. Excel spreadsheet showing all the proteins identified among the affinity-purified targets of 147.
elife-37168-table1-data2.xlsx (84.7KB, xlsx)
DOI: 10.7554/eLife.37168.010
| Protein name | Symbol | Uniprot accession | Competition Ratio* | Redox?† | ER-localization?‡ |
|---|---|---|---|---|---|
| Cytoskeleton-associated protein 4 | CKAP4 | Q07065 | 2.8 (1.7, 3.5) | N | Y |
| Protein disulfide isomerase A4 | PDIA4 | P13667 | 2.4 (1.8, 3.3) | Y | Y |
| Protein disulfide isomerase A6 | PDIA6 | Q15084 | 2.4 (2.0, 3.0) | Y | Y |
| Protein disulfide isomerase A1 (a.k.a. Prolyl 4-hydroxylase subunit beta) | PDIA1 (a.k.a. P4HB) | P07237 | 2.3 (2.1, 3.1) | Y | Y |
| Protein disulfide isomerase A3 | PDIA3 | P30101 | 2.2 (1.8, 3.1) | Y | Y |
| Thioredoxin | TXN | P10599 | 2.2 (1.2, 3.5) | Y | N |
| Thioredoxin domain-containing protein 5 | TXNDC5 | Q8NBS9 | 2.2 (2.0, 2.8) | Y | Y |
| Heme oxygenase 2 | HMOX2 | P30519 | 2.2 (1.4, 4.3) | N | Y |
| Chloride intracellular channel 1 | CLIC1 | O00299 | 1.9 (1.5, 2.3) | N | N |
| Thioredoxin-related transmembrane protein 1 | TMX1 | Q9H3N1 | 1.9 (1.5, 4.3) | Y | Y |
| Voltage dependent anion channel 2 | VDAC2 | P45880 | 1.6 (1.2, 1.9) | N | N§ |
| Poly(rC)-binding protein 1 | PCBP1 | Q15365 | 1.6 (1.4, 1.8) | N | N |
| Ribosome-binding protein 1 | RRBP1 | Q9P2E9 | 1.5 (1.3, 1.8) | N | Y |
| Proteasome subunit alpha type 1 | PSMA1 | P25786 | 1.5 (1.1, 1.7) | N | N |
| d-3-phosphoglycerate kinase | PHGDH | O43175 | 1.5 (1.4, 1.9) | N | N |
| Histocompatibility minor 13 | HM13 | Q8TCT9 | 1.5 (1.2, 2.0) | N | Y |
| Polyadenylate-binding protein 4 | PABPC4 | Q13310 | 1.5 (1.1, 1.7) | N | N |
| Ribophorin 1 | RPN1 | P04843 | 1.4 (1.3, 1.8) | N | Y |
| ER resident protein 29 | ERP29 | P30040 | 1.4 (1.0, 1.8) | N | Y |
*Competition ratio = Ratio of TMT reporter ions for the protein in question between samples treated with 147–20 and those co-treated with 147–20 and 147. The median value across all cell lines and replicates are shown, with the values at the 1st and 3rd quartiles shown in parenthesis.
†Indicates whether the protein in question has the ‘cell redox homeostasis’ GO annotation (GO:0045454).
‡Indicates whether the protein in question has the ‘endoplasmic reticulum part’ GO annotation (GO:0044432).
§VDAC2 is a mitochondrial membrane protein but has been shown to localize to sites of mitochondria that are associated with the ER.