Table 1.

Primers expected molecular weight of PCR products and PCR conditions for B1 gene.

Primers	Sequence (5′-3′)	Size (bp)	PCR conditions
First round
OB1/F	5′-GGAACTGCATCCGTTCATGAG-3′	200 bp	950°C for 5 mins followed by 40 cycles (950°C for 30 secs, 560°C for 30 secs, 720°C for 30 secs.
OB1/R	5′-TCTTTAAAGCGTTCGTGGTC-3′
Second round
IB1/F	5′-TGCATAGGTTGCAGTCACTG-3′	100 bp	950°C for 5 mins followed by 35 cycles (950°C for 30 secs, 560°C for 30 secs, 720°C for 30 secs.
IB1/R	5′-GGCGACCAATCTGCGAATACACC-3′