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. 2001 Oct 30;98(23):12984–12989. doi: 10.1073/pnas.241175798

Figure 1.

Figure 1

Heparan sulfate and chimeric enzymes. (A) Schematic representation of heparan sulfate. The arrows indicate the sites of action for the enzymes examined in this study. NDST, N-deacetylase/N-sulfotransferase 1. (B) To localize the enzymes, green fluorescent protein (GFP) was spliced on the C termini of the enzymes. To relocate the enzymes, the cytoplasmic tail of the enzymes was replaced with the endoplasmic reticulum (ER)-retention domain from human invariant chain (p33). TM, transmembrane domain.