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Golgi localization of Epi depends on the presence of 2OST. pgsF17 cells lacking endogenous 2OST were transiently transfected with NDST1-GFP (A), 2OST-GFP (B), or Epi-Myc (C). NDST1 and 2OST colocalized with the α-mannosidase marker, whereas Epi-Myc was found in the ER. In D, the mutant was stably transfected with 2OST cDNA to the correct the enzyme deficiency, and then transiently transfected with 2OST-GFP or Epi-Myc.
