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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Matrix Biol. 2018 Mar 9;70:20–35. doi: 10.1016/j.matbio.2018.03.009

Fig 5. β6 integrin down-regulation results in increased expression of STAT1 and MX1/2 in PC3 cells and exosomes.

Fig 5

(A), IB analysis of STAT1 expression in 30 μg of exosome lysate (Exo) and total cell lysate (TCL) derived from PC3 cells incubated either with a non-silencing siRNA (NS siRNA) or with β6 mRNA directed siRNA (D1 or D2). ERK was used as loading control for TCL and TSG101 was used as loading control for Exo lysate. (B), IB analysis of the expression of exosomal markers CD81, CD9, CD63 in PC3 cell Exo lysate and TCL derived from PC3 cells incubated either with a NS siRNA or with D1 and D2 siRNA. CANX was used as loading control found in TCL but not in Exo. (C), IB analysis of the expression of β6 integrin and MX1/2 in TCL and Exo derived from parental PC3 cells or PC3 cells transfected with shβ6 or shβ5 retroviral constructs. PC3-shβ5 transfectants are used as a negative control. CANX was used as a loading control for TCL and CD9 was used as a loading control for Exo lysates.