Fig 7. α_vβ₆ Integrin is expressed in PBMC from prostate cancer patients.

(A), Flow cytometric analysis of α_vβ₆ expression in PBMC from prostate cancer patients and healthy subjects. Left, monocytes and lymphocytes are gated by SSC and FSC. Right, FACS analysis of α_vβ₆ cell surface expression in monocytes and lymphocytes from healthy subjects and prostate cancer patients respectively, utilizing 6.3G9 monoclonal antibody to α_vβ₆ and mouse IgG, as isotype control. Representative data are shown. (B), The schematic diagram shows that transfer of exosomal α_vβ₆ integrin from prostate cancer cells to monocytes results in down-regulation of STAT1 and MX1/2 levels and increased M2 polarization of monocytes, and has a pro-tumorigenic effect, whereas transfer of α_vβ₆ integrin–negative exosomes results in increased M1 polarization of monocytes, and inhibits cancer growth