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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Eur Urol Focus. 2018 Feb 7;5(4):664–675. doi: 10.1016/j.euf.2018.01.017

Fig. 3.

Fig. 3

Testing of specificity for chromosomal FISH probes selected to design the quartet test. (A) Dual color FISH with centromeric and respective chromosomal probes 6p22 E2F3, 8q22 YWHAZ, 11q13 FGF3, and 20q11 MAPRE13. (B) Hybridization signals for a mixture of four probes (6p22, E2F3, green, PlatinumBright495; 8q22, YWHAZ, gold, PlatinumBright530; 11q13, FGF3, dark red, PlatinumBright590; and 20q11, MAPRE13, blue, PlatinumBright415) showing the hybridization signals on their respective human metaphase chromosomes. (C) Hybridization signals obtained with a mixture of the four probes listed above (the quartet test) in normal human urothelial cells. (D) Hybridization signals obtained with a mixture of four probes comprising the quartet test in a cell from a touch print prepared from a tumor tissue of high-grade bladder cancer.

FISH = fluorescence in situ hybridization.