Fig. 5.

Promoter-swap analysis of Sus3. (A)Vector constructs for promoter-swap analysis. Promoter sequences of Sus3^Koshi (pKoshi) and Sus3^Haba (pHaba) were fused to the coding cDNA sequences of Sus3^Koshi (cKoshi) and Sus3^Haba (cHaba). All four constructs were fused with the Nos terminator at the end of the coding sequence. (B) High-temperature treatment test of promoter-swap plants. The four constructs indicated in (A) were transformed into ‘Nipponbare’. Transformants were grown under high-temperature condition during the ripening stage. The percentages of basal-white and white-back kernels were measured to compare high-temperature tolerance. Error bars indicate standard deviations. Different letters above the bars indicate a significant difference at 5% (Tukey’s HSD test), n > 3.