Figure 1.

CDX2 stimulates ST14 mRNA expression and inhibits SPINT1 mRNA expression in intestinal epithelial cells. In the absence of doxycycline (−Dox), the LS174T intestinal cell line with Tet-On inducible system to control CDX2 expression has knocked-out the endogenous CDX2 genomic locus using a Tet3G transactivator element (described in³²). Treatment with doxycycline (+Dox) stimulates ectopic CDX2 expression from the Dox-inducible cassette. Experiments are compared to wild-type (wt) LS174T cells harboring no Tet-On system. Relative gene expression of ST14 and SPINT1 were normalized to β-Actin mRNA levels. Data are expressed as mean values ± S.E.M (n = 4), *P < 0.05 (one-way ANOVA analysis).