Figure 4.

ST14 and SPINT1 enhancers contain several predicted CDX2-binding sites. In silico sequence analysis of the predicted human ST14 enhancer, position +21579 to +22159 (580 bp), and of the SPINT1 enhancer, position +3331 to +3863 (532 bp), relative to the transcriptional start site, shown in (a and b) respectively. The search for transcription factor binding sites was performed using the Transfac database. Predicted CDX2 binding sites are marked in white, GATA4 binding in green and HNF4α binding in blue. The indicated “F primer” and “R primer” are the forward and reverse primer pairs that have been used to amplify CDX2 bound regions in the ChIP-PCR assay in Fig. 2(c) and (d). The human sequences are aligned to the mouse genome using Clustal W analysis and conserved nucleotides are shown in red.