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. 2018 Aug 7;8:11813. doi: 10.1038/s41598-018-30216-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CDX2 regulates ST14 and SPINT1 reporter constructs in intestinal cells. ST14 (a) and SPINT1 (b) reporter plasmids were analyzed in intestinal RKO and LS174T cells. RKO cells were transiently transfected with or without overexpression of CDX2. For the LS174T cells, wild type cells were compared to cells with doxycycline-regulated CDX2 levels. (a) The ST14 promoter and promoter/enhancer activity were stimulated by CDX2 in RKO cells. In LS174T cells, the opposite is observed where CDX2 knock-out (−CDX2) resulted in increase of ST14 promoter and enhancer activity. (b) CDX2 did not affect SPINT1 promoter activity, but reduced SPINT1 enhancer activity. In LS174T cells loss of CDX2 (−CDX2) resulted in increased SPINT1 and enhancer activity. The reporter activity was normalized to the co-transfected LacZ plasmid expression, (mean values presented as S.E.M, two-way ANOVA analysis) n = 4 (****P < 0.0001).