Fig. 4.
Expression of GABAAR α1 and α2 subunits, and related inhibitory synaptic proteins in Gabra2–1 mice. a Representative images of GABAAR α2-subunit staining in the CA1 region of the hippocampus. Quantification of the size: (b WT—0.365 ± 0.004; Het—0.279 ± 0.003; Homo—0.271 ± 0.003), and density (c WT—192.80 ± 7.851; Het—202.70 ± 17.490; Homo—347.20 ± 5.276) of α2 positive clusters in CA1. d Quantification of GABAAR α1 subunit cluster density (WT—145.00 ± 17.50; Het—184.10 ± 6.31; Homo—165.00 ± 28.33) in CA1. e Cluster size/intensity correlation plots for α2 and α1 staining, showing the shift toward smaller, less intense clusters of α2 in Gabra2–1 homozygous mice. f Representative images of CB immunostaining in CA1. g Representative images of VGAT immunostaining in CA1. Quantification of the size (h WT—0.344 ± 0.024; Het—0.232 ± 0.035; Homo—0.188 ± 0.020) and density i WT—3056.06 ± 338.30; Het—1913.26 ± 275.02; Homo—1087.94 ± 165.43) of CB positive clusters in CA1. j Quantification of VGAT positive cluster density (WT—3447.82 ± 250.00; Het—3165.59 ± 390.82; Homo—3346.53 ± 289.30) in CA1. Scale bar = 10 µm, applies to all images. All plots shown and all values listed are mean ± standard error, p values from KS test (cluster size), or ANOVA (cluster density)