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. 2018 Aug 7;9:3126. doi: 10.1038/s41467-018-05616-4

Fig. 5.

Fig. 5

The role of candidate motifs on hBest1 in ATP-dependent activation. a Left, representative current traces recorded from hBest1 P274R iPSC-RPE. Right, representative current traces in hBest1 P274R iPSC-RPE complemented with WT hBest1-GFP (Scale bar, 1.5 nA, 100 ms). Insert, confocal images showing expression of WT hBest1-GFP in rescued P274R iPSC-RPE (Scale bar, 10 μm). b Population steady-state current–voltage relationships in P274R iPSC-RPE, and in P274R iPSC-RPE complemented with WT hBest1-GFP in the absence or presence of 2 mM ATP, n = 5–6 for each point. *#P < 0.05 compared to uninfected cells and complemented cells in presence of ATP, respectively, using two-tailed unpaired Student t test. c Bar chart showing the steady-state current amplitudes of P274R iPSC-RPE complemented with WT or mutant hBest1 channels, n = 5–6 for each bar. #*P < 0.05 compared to currents from the same set of cells in the presence of 2 mM ATP, respectively, using two-tailed unpaired Student t test. All error bars in this figure represent s.e.m.