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. 2018 Aug 7;8:11849. doi: 10.1038/s41598-018-30329-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Design of the oxygen sensor protein probe. Primary structures of protoANA1 (A) and protoANA2 (B) are indicated. A variant of YFP (Venus) and the oxygen sensor domain of DosP from E.coli (DosH) were conjugated with a Gly-Ser linker or an antiparallel coiled-coil linker (APC linker), respectively. Fluorescence quenching mechanisms are schematically shown for protoANA1 (C), protoANA2 (D), ANA-G (E) and ANA-Q (F). In the oxygen free form, acceptor DosH exhibited a visible absorption and efficiently quenched fluorescence from donor Venus. In the oxygen-bound form, DosH had reduced absorption, leading to increased fluorescence emissions from Venus.