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. 2018 Aug 7;8:11849. doi: 10.1038/s41598-018-30329-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spectroscopic properties of ANA-G. (A) Excitation (gray color) and emission (black color) spectra of ANA-green (ANA-G; 1 μM) were measured in the absence (dashed line) or presence (solid line) of 240-μM oxygen. Excitation and emission spectra of ANA-G were measured at emission and excitation wavelengths of 510 and 490 nm, respectively. (B) Emission spectra of ANA-G His348Ala variant (1 μM) in the absence (dashed line) or presence (solid line) of 240-μM oxygen; Data are presented relative to the fluorescence intensity of ANA-G in the presence of oxygen (1.0).