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. 2018 Aug 7;8:11849. doi: 10.1038/s41598-018-30329-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Monitoring of photosynthetic oxygen production in cyanobacteria using ANA sensors. Time courses of changes in emission intensities of (A) ANA-Y, (B) ANA-G and (C) ANA-Q solution suspending A.7120 cells; suspensions of A.7120 cells (1.2–2.4 μg Chl mL⁻¹) in the presence (diamond) or absence (circle) of 10-μM DCMU (ANA-Y) or 12.5-μM DCMU (ANA-G or ANA-Q) were illuminated at 13 μmol photons m⁻² s^−l and emission intensities of ANA-Y (1.7 μM) at 527 nm, ANA-G (1.7 μM) at 510 nm and ANA-Q (1.0 μM) at 527 nm and 602 nm were measured. Solution without A.7120 cells were used as a negative control (square). Time courses of emission intensity changes of Venus in A.7120 cells suspension was measured as a control (A, triangle). Fluorescence intensity data are presented relative to that of ANA-Y or ANA-G at time 0 (1.0), or fluorescence ratio of F527/F602. All data represent means ± s.d. from three replicates.