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. 2018 Jul 24;2018:6712585. doi: 10.1155/2018/6712585

Figure 7.

Figure 7

DS-201 prevents Ang II-induced TGF-β1 activation in human atrial fibroblasts. Atrial fibroblasts were exposed to Ang II (0.5 μM) with or without DS-201 (0, 5, 25, 50, and 100 μM) for 24 h. (a) Expression of TGF-β1 was analyzed by western blotting, and representative images of 3 independent experiments are shown. The ratio of TGF-β1 normalized to GAPDH was calculated. (b) Phosphorylation (p) of Smad2/3 was analyzed by western blotting. Representative images of 3 independent experiments and densitometric analysis of phosphorylated Smad2/3 normalized to total Smad2/3 are shown. (c) Expression of periostin was analyzed by western blotting, and representative images of 3 independent experiments are shown. The ration of periostin normalized to GAPDH was calculated. ∗∗ P < 0.01 versus control (first bar); ## P < 0.01 versus Ang II.