Figure 5.

Salidroside (SAL) increases phosphorylation of AMPK, ACC, Akt, and GSK3β and suppresses activation of NLRP3 inflammasome in HFD mice. After treatment with SAL (100 mg·kg⁻¹·d⁻¹) for 8 weeks, liver tissues were obtained from RD and HFD mice. For the assessment of insulin sensitivity in vivo, mice were stimulated with 0.75 mU·g⁻¹ insulin for 10 min after a 12 h fast before sacrificed. The phosphorylation of AMPK, ACC (a), Akt, and GSK3β (b) and the activation of NLRP3 inflammasome (c) were analyzed by immunoblot. ^† P < 0.05, ^†† P < 0.01 versus RD mice treated with vehicle; ^∗ P < 0.05, ^∗∗ P < 0.01 versus HFD mice treated with vehicle. Values are means ± s.e.m. (n = 3).