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Reactive oxygen species (ROS) production in cultured primary neocortical neurons. (a) Neurons were pretreated with NSC-CM and Ly-NSC-CM for 4 hours, then exposed to t-BHP (10 μM) for 24 hours. The generation of ROS was detected by flow cytometry. (b) The ROS levels were assayed by measuring the fluorescence intensity of DCF. Mean ± SEM. n = 3 per group. ∗P < 0.05 versus control group. #P < 0.05 versus t-BHP group. &P < 0.05 versus NSC-CM group.
