Figure 5.

Induction of −6R15/CAT and −6R15/PyLT transgene expression by β-receptor agonist, isoproterenol, and high-tannin sorghum. Results from representative independent lines containing either −6R15/CAT or −6R15/PyLT transgene are shown. A. CAT assays were performed using 100 μg of soluble protein at 37°C for 14 hours. The percent conversions were Ipr-Pa, 23%; Tannin-Pa, 62%; <1% for the remaining tissue extracts. B. Northern blot was first probed with PyLT probe for transgene expression (upper panel), and then the same blot was stripped and rehybridized with mouse β-actin cDNA probes (lower panel). The autoradiography was performed with double intensifying screens at −80°C for 4 days (PyLT) and 2 days (β-actin), respectively. The positions of 18S ribosomal RNA, as visualized by EtBr staining, are shown at the right. See Figures 2 and 3 for further details.