Table 1.

Effect of mutations on GABP binding and rpL30/rpL32 promoter activity.

Binding was assayed by EMSA, as shown in Figure 5. For activity measurements, the rpL30 and rpL32 promoters (−205 to +167 and −159 to +115, respectively) containing wild-type or mutant GABP binding sites were linked to a cat reporter plasmid and transfected into SI 94 plasmacytoma cells. Extracts of cells harvested 40 hours after transfection were assayed for CAT activity and normalized to the values for the wild-type promoter. The mean values for duplicate assays of two to five independent transfection experiments are listed together with the average deviations. For ease of comparison of common motifs, the sequences on the minus strand of rpL30 and the plus strand of rpL32 are listed.
Gene	Proximal(B) site | distal(A) site	Binding complex		Activity
		αβ1	(αβ1)2
rpL30
wild-type	5′-CGGAAGCGGAAG-3′	++	++	100
A1 mutant	CGGAAGCGGAgG	++	−	78 ± 18
A2 mutant	CGGAAGCGGcAG	++	−	69 ± 8
B mutant	CGGcAGCGGAAG	+	−	42 ± 13
AB mutant	CGGcAGCCCAgG	−	−	27 ± 7
rpL32
wild-type	5′-CGGAAG-3′	++	−	100
mutant	CtGAAG	−	−	44 ± 8