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Vector for the expression of p53 and domains of p53. The vector contains the MSV promoter, a cassette for insertion of protein segments, an RNA splice signal, and a polyadenylation signal. Insertion of coding sequences in-frame in the cassette creates a tagged protein. The tag contains an optional E1A nuclear localization signal (NLS), six histidines for metal affinity purification, the KT3 epitope, and a site for cleavage by proteinase Xa.
