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. 2018 Aug 8;3(2):175–185.

Figure 4.

Figure 4

Transcription analysis from the 7SK and U6 core promoters either separately (lanes 1–4) or simultaneously in the same reaction (lanes 5–8). Transcription was either with the phosphocellulose B fraction alone (lanes 1, 3, 5, 7) or with the B + C combination (lanes 2, 4, 6, 8). Templates used were 1.0 μg of the 7SK construct (lanes 1, 2) or the U6 construct (lanes 3, 4). In lanes 5 to 8, 0.5 μg of both constructs were transcribed simultaneously, under conditions optimal either for 7SK (lanes 5, 6) or for U6 (lanes 7, 8) RNA transcription in vitro. Analysis of transcripts with S1 nuclease was as above.