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Isothermal calorimetry data for holoRBP (980 μM) titrated into wild-type TTR (50 μM)‡. The isotherm was fit to a model of equivalent sites by using the variables Ka, ΔH, and n (Kd is determined as the inverse of Ka). Values from 6 titrations were averaged to yield Kd = 294 ± 165 nM (ΔG = −8.837 ± 0.337 kcal/mol), ΔH = −0.884 ± 0.191 kcal/mol, and the number of sites n = 2.110 ± 0.048.
