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Sedimentation velocity data for wild-type TTR (7.2 μM)‡ and holoRBP (7.2 μM) at pH 7.6. The data were fit to a three-species model of TTR⋅holoRBP, TTR⋅holoRBP2, and holoRBP monomer. More than 80% of holoRBP was complexed at pH 7.6, yielding a Kd lower than 7.2 μM.
