Figure 5.

Time course of wild-type (pH 4.4) (A and C) and L55P TTR (pH 5.0) (B and D) fibril formation. TTR (3.6 μM)^‡ was incubated with holoRBP (7.2 μM). Fibril formation was quantified by two techniques at each timepoint: turbidity at 400 nm (A and B) and Congo red binding (C and D). Fibril formation from TTR alone is shown by diamonds, fibril formation from the TTR⋅holoRBP complex is shown by circles, and precipitation from holoRBP is shown by squares.