TABLE 1 .
Volatile fatty acid (VFA) concentrations of all rumen fluid samples taken between 4 a.m. and 4 p.m.
| Cow | Time | VFA concn (nmol·g−1 rumen fluid) |
pH | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Formic acid |
Acetic acid |
Propionic acid |
Iso- butyric acid |
Butyric acid |
Iso- valeric acid |
Valeric acid |
Caproic acid |
Heptanoic acid |
Lactic acid |
Succinic acid |
Total VFAs |
|||
| 1 | 04 a.m. | 0.0 | 63.4 | 16.8 | 0.8 | 16.2 | 0.5 | 1.8 | 0.2 | 0.0 | 0.0 | 0.0 | 99.7 | |
| 07 a.m. (t0) | 0.0 | 62.4 | 15.4 | 0.9 | 14.9 | 0.6 | 1.6 | 0.2 | 0.0 | 0.0 | 0.0 | 95.9 | 6.67 | |
| 08 a.m. (t1) | 0.0 | 61.6 | 19.6 | 0.8 | 15.2 | 0.6 | 1.5 | 0.2 | 0.0 | 9.1 | 0.1 | 108.8 | 6.22 | |
| 10 a.m. (t3) | 0.0 | 67.2 | 20.7 | 1.0 | 16.7 | 0.6 | 2.2 | 0.2 | 0.0 | 0.3 | 0.0 | 108.8 | 6.22 | |
| 12 a.m. (t5) | 0.0 | 54.3 | 13.4 | 0.8 | 11.9 | 0.4 | 1.3 | 0.1 | 0.0 | 0.0 | 0.0 | 82.1 | 6.56 | |
| 02 p.m. | 0.0 | 55.9 | 13.0 | 0.8 | 11.0 | 0.4 | 1.0 | 0.1 | 0.0 | 1.5 | 0.0 | 83.8 | ||
| 04 p.m. | 0.0 | 59.3 | 22.6 | 0.8 | 15.9 | 0.7 | 1.7 | 0.2 | 0.0 | 0.9 | 0.0 | 102.1 | ||
| 2 | 04 a.m. | 0.0 | 49.7 | 23.3 | 0.8 | 7.3 | 0.4 | 3.0 | 0.5 | 0.1 | 0.0 | 0.0 | 85.1 | |
| 07 a.m. (t0) | 0.0 | 56.7 | 24.4 | 0.9 | 7.3 | 0.4 | 2.7 | 0.5 | 0.1 | 0.0 | 0.0 | 93.1 | 6.68 | |
| 08 a.m. (t1) | 0.0 | 55.7 | 21.6 | 0.9 | 11.4 | 0.5 | 4.2 | 1.0 | 0.1 | 0.9 | 0.0 | 96.2 | 6.48 | |
| 10 a.m. (t3) | 0.0 | 66.1 | 30.6 | 1.0 | 12.8 | 0.6 | 4.9 | 1.2 | 0.2 | 0.0 | 0.0 | 117.3 | 6.22 | |
| 12 a.m. (t5) | 0.0 | 60.1 | 27.7 | 1.0 | 9.3 | 0.5 | 3.4 | 0.8 | 0.1 | 0.0 | 0.0 | 102.8 | 6.54 | |
| 02 p.m. | 0.0 | 59.7 | 24.4 | 0.9 | 8.6 | 0.5 | 2.9 | 0.6 | 0.1 | 6.8 | 0.2 | 104.7 | ||
| 04 p.m. | 0.3 | 54.2 | 23.0 | 0.7 | 15.3 | 0.5 | 4.9 | 1.4 | 0.1 | 16.2 | 0.2 | 116.7 | ||
| 3 | 04 a.m. | 0.0 | 59.9 | 24.8 | 0.7 | 10.6 | 0.4 | 2.1 | 0.5 | 0.1 | 0.0 | 0.0 | 99.0 | |
| 07 a.m. (t0) | 0.0 | 54.7 | 18.7 | 0.8 | 8.6 | 0.3 | 1.3 | 0.3 | 0.0 | 0.0 | 0.0 | 84.8 | 6.84 | |
| 08 a.m. (t1) | 0.0 | 59.4 | 21.8 | 0.9 | 12.0 | 0.5 | 2.0 | 0.6 | 0.0 | 0.0 | 0.0 | 97.2 | 6.46 | |
| 10 a.m. (t3) | 0.0 | 61.3 | 23.0 | 0.8 | 11.1 | 0.4 | 1.7 | 0.5 | 0.0 | 0.0 | 0.0 | 98.9 | 6.47 | |
| 12 a.m. (t5) | 0.0 | 60.3 | 21.1 | 0.9 | 10.0 | 0.5 | 1.4 | 0.4 | 0.0 | 0.0 | 0.0 | 94.6 | 6.76 | |
| 02 p.m. | 0.0 | 62.6 | 20.9 | 0.9 | 11.3 | 0.5 | 1.3 | 0.4 | 0.0 | 8.3 | 0.1 | 106.2 | ||
| 04 p.m. | 0.0 | 65.5 | 29.1 | 1.0 | 17.6 | 0.8 | 2.4 | 0.8 | 0.0 | 1.7 | 0.0 | 118.9 | ||
| 4 | 04 a.m. | 0.0 | 70.2 | 32.3 | 0.8 | 12.4 | 0.5 | 3.2 | 0.4 | 0.0 | 0.0 | 0.0 | 119.9 | |
| 07 a.m. (t0) | 0.0 | 56.1 | 21.4 | 0.7 | 8.0 | 0.3 | 1.7 | 0.2 | 0.0 | 0.0 | 0.0 | 88.4 | 6.52 | |
| 08 a.m. (t1) | 0.0 | 54.2 | 18.9 | 0.8 | 8.1 | 0.3 | 1.5 | 0.2 | 0.0 | 0.0 | 0.1 | 84.0 | 6.71 | |
| 10 a.m. (t3) | 0.0 | 60.8 | 20.0 | 0.9 | 7.8 | 0.4 | 1.3 | 0.2 | 0.0 | 0.0 | 0.0 | 91.4 | 6.71 | |
| 12 a.m. (t5) | 0.0 | 53.0 | 15.8 | 0.9 | 6.1 | 0.4 | 0.8 | 0.1 | 0.0 | 0.0 | 0.0 | 77.0 | 6.91 | |
| 02 p.m. | 0.0 | 53.5 | 15.5 | 0.9 | 8.7 | 0.4 | 1.2 | 0.2 | 0.0 | 4.9 | 0.0 | 85.2 | ||
| 04 p.m. | 0.0 | 64.5 | 24.8 | 0.9 | 21.6 | 0.7 | 3.5 | 0.6 | 0.0 | 10.7 | 0.0 | 127.3 | ||