Table 2.

Activities of Methanococcus DHBPS mutants

		% of wild-type activity
	Wild type	100
Region I	D33S	n.d.
	D35S	16
	R37E	n.d.
	E38S	n.d.
	E40S	n.d.
	D42S	n.d.
Region II	C67S	19
Region III	T107S	n.d.
	T110A	16
	D113S	12
	T117A	26
Region IV	H136S	n.d.
Region V	R150S	n.d.
	H153S	n.d.
	T154A	32
	E155S	17
Region V	E174S	n.d.

All numbering based on E. coli DHBPS (see Fig. 3). n.d., activities were below the detection limit of the assay.