Fig. 1.

The lentiviral vector expressing miR-125a or miR-205 was constructed. a Map of the lentiviral vector pCDH-CMV-MCS-EF1-Puro. b Primers used in PCR assays to amplify the precursor of miR-125a or miR-205. The capital letters in red and blue indicate the digested sites of Xba I and Not I, respectively. c Electrophoretogram of the PCR products and the lentiviral vector digested with Xba I and Not I. d Strategy to construct the lentiviral vector with miRNA expression. e Results of PCR and DNA sequencing analysis of positive colonies