Fig. 5.

Co-expression of miR-125a and miR-205 via cluster transfection profoundly enhanced trastuzumab-induced growth inhibition and cell cycle G1 arrest in BT474 cells. The stable sub-clones of BT474 cells treated with trastuzumab (20 μg/ml) for 48 h were collected and subjected to cell cycle analysis by flow cytometry (a) and cell proliferation (the IncuCyte system) (b). c The stable sub-clones of BT474 cells treated with trastuzumab (20 μg/ml) for 24 h were collected and subjected to western blot analysis with the specific antibodies directed against E2F1, Cyclin D1, p27^kip1 (p27), or β-actin