Fig. 6.

The miRNA cluster transfection significantly increased paclitaxel-mediated cytotoxicity effects on HCC1954 breast cancer cells. a & b The stable sub-clones of HCC1954 cells transfected with empty vector (pCDH), pCDH-miR-125a, or pCDH-miR-205 alone or pCDH-miR-125a-miR-205 (cluster) were selected. The relative expression levels of miR-125a and miR-205 were measured by qRT-PCR using TaqMan miRNA assays. c Proliferation of the sub-clones was measured by analysis of the IncuCyte system. d The stable sub-clones were collected and subjected to western blot analyses of p-erbB3, HER3 (erbB3), p-Akt, Akt, or β-actin. e & f Each sub-clone treated with paclitaxel (10 nmol/L) for 24 h were collected and subjected to apoptotic-ELISA (e) or western blot analyses with specific antibodies directed against (F-PARP, full length PARP; C-PARP, cleaved PARP), caspase-8 (F-Casp-8, full length caspase-8; C-Casp-8, cleaved caspase-8), caspase-3 (F-Casp-3, full length caspase-3; C-Casp-3, cleaved caspase-3), or β-actin (f). Bars, SD. Data shows the representative of three independent experiments