Fig. 1.

dsrD phylogenetic affiliation and abundance per sample. The RAxML tree was constructed using 206 amino acid sequences. The gene or gene cluster (C1–23) affiliation was inferred from the (representative) best BLASTP hit. Bolded names represent dsrD present in reconstructed genomes. The yellow, blue, and orange stars indicate dsrD in genomes represented in Fig. 2. For the heat map, dsrD-containing contig RPKM values were used as input. Clusters are represented by the sum of RPKM values. The statistical significance of hierarchical clustering branches is indicated by green stars (pvclust, approximately unbiased p < 0.05). Additional file 4: Figure S1 is an expanded version of this figure, displaying each one of the 206 sequences