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. 2018 Aug 8;15:17. doi: 10.1186/s12979-018-0122-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Distribution of peripheral CD8 T cells in naive young and aged mice. a Representative flow cytometry staining from spleen illustrating the gating strategy for identifying CD8 subsets in B and C. b The distribution of naïve (CD62L^High/CD44L^Low), CM (CD62L^High/CD44^High) and EM (CD62L^Low/CD44^High) in the spleen and lung of young (2–3 months) and aged (18–22 months) SPF mice. c The number of VM (CD49d^Low) and TM (CD49d^High) among the CM population in the spleen and lung of young and aged mice. Bars represent the mean ± SD. The data are from a single experiment, n = 5 mice/group, and are consistent with published data [42]. Data were analyzed by unpaired, two-tailed T test (**p < 0.01)