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. 2018 Aug 7;11:65. doi: 10.1186/s13048-018-0437-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Sheep oocytes after the live-dead assay. Oocyte were derived from the follicles which were subsequently mechanically isolated, frozen and warmed/thawed. Viability of oocytes were evaluated in staining by calcein-am and ethidium homodimer-1. Letter “a” shows alive oocyte after freezing/thawing showed, “b” - degenerated oocyte