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. 2018 Aug 7;18:130. doi: 10.1186/s12890-018-0700-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Shp2 inhibition ameliorates muscularization of pulmonary arterioles in MCT induced PAH rats. a Phps-1 decreased α-SMA expression of lungs in MCT induced PAH. Representative images of immunofluorescent stainings for CD31 (red) and α-SMA (green) in lungs were showed. Nucleus (blue) was stained with DAPI. Scale bar = 50 μm. b The muscularization of distal pulmonary arterioles was determined by calculating the percent of arteries that were fully (> 75%), partially (25–75%) and not muscularuized (< 25%) (n = 4–6). Data was presented as means ± standard deviation (SD), *** P < 0.001