TABLE 1.
Antibodies | Vendors | Catalog number | Dilution | Source | Specificity |
---|---|---|---|---|---|
PDGFR β | Abcam, Cambridge, MA | ab32570 | 1:50(with 1 % BSA-PBS) | Rabbit | Mouse, rat, human |
PDGF-BB | Abcam, Cambridge, MA | ab181341 | 1:50 (with 1 % BSA-PBS) | Rabbit | Mouse, rat, human |
Desmin | Abcam, Cambridge, MA | ab32362 | 1:50 (with 1 % BSA-PBS) | Rabbit | Mouse, rat, human |
GAPDH | Santa Cruz Biotechnology | sc-20,357 | 1:200(with 1 % BSA-PBS) | Goat | Mouse, rat, human |
Peroxidase-labeled anti-rabbit antibody | GE healthcare Bio-Sciences, Pittsburgh, PA | NIF 824 | 1:5000(with 5 % skim milk) | Donkey | Goat |
Donkey anti-goat IgG-HRP | Santa Cruz Biotechnology, Dallas, TX |
SC-2020 | 1:5000(with 5 % skim milk) | Donkey | Goat |
Alexa Fluor 488-conjugated goat anti-rabbit IgG | Invitrogen, Eugene, OR | A-11008 | 1:100(with 1 % BSA-PBS) | Goat | Rabbit |