Fig. 5.

GLAST-CreER^T2 mice have inducible Cre activity in supporting cells. a Confocal images showing immunolabeling for GLAST (red), myosin VIIa (Myo, green), and nuclei (DAPI, blue) in slices focused on the type II hair cell nuclear layer. b GLAST labeling only in same field as shown in a. c-d Confocal images of the utricle from GLAST-CreER^T2:ROSA26^tdTomato mice injected with tamoxifen, focused on either the macula (c,c') or the stroma (d). c and c' show the same view, with labeling for tdTomato (Tom, red), myosin VIIa (Myo, green), and DAPI (blue) shown in c and Tom only shown in c'.S = approximate position of the striola, TE = transitional epithelium. e Utricle from a GLAST-CreER^T2:ROSA26^tdTomato mouse that did not receive tamoxifen, focused on the sensory epithelium. f-g Confocal slices through the lateral extrastriolar region, with slices through the type II hair cell (HC) nuclear layer f), the type I hair cell (HC) nuclear layer (f'), the supporting cell (SC) nuclear layer (f"), or the stroma (g). Scale bar in a = 5 μm and applies to a,b. Scale bar in c = 150 μm and applies to c-e. Scale bar in f = 5 μm and applies to f-g