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. 2018 Mar 14;70(4):1155–1165. doi: 10.1007/s10616-018-0206-8

Table 3.

Primer sequences of the stemness markers

Gene name NCBI accession Sequence Product (bp)
Oct4 XM_013977063.1 Forward 5′-GCCAAGCTCCTAAAGCAGAAGA-3′ 122
Reverse 5′-AAAGCCTCAAAACGGCAGATAG-3′
Nanog NM_001285576.1 Forward 5′-GTCTCTCCTCTTCCTTCCTCCA-3′ 116
Reverse 5′-TCTTCCTTCTCTGTGCTCTCCTC-3′
Sox2 NM_001285672.1 Forward 5′-CATGATGGAGACGGAACTGG-3′ 115
Reverse 5′-CGGGCTGTTCTTCTGGTTG-3′
AKP XM_013974604.1 Forward 5′-ATGGTCACCATGAAGGCAAAG-3′ 125
Reverse 5′-ATGGTCTGCAGTGGCAAGGA-3′
TERT XM_013972889.1 Forward 5′-GTGCTGAACTACGAGCGAGC-3′ 147
Reverse 5′-GTCCGCCTTGACGAAGTAGAG-3′
GAPDH XM_005680968.2 Forward 5′-TTGTGATGGGCGTGAACC-3′ 127
Reverse 5′-CCCTCCACGATGCCAAA-3′