Fig. 5.

Co-culture systems promote HNF4α expression of hepatocytes. a Representative confocal immunofluorescence images of HNF4α expression in hepatocytes after co-culturing for 7 days (magnification, ×40; scale bar, 50 μm). HUVECs are stained with DsRed; LO2 cells are stained with EGFP. Co-culture systems significantly enhanced HNF4α expression in hepatocytes compared with those in the monoculture. b Fluorescence intensity of ALB expression. c Fluorescence intensity of EGFP (LO2). For all groups, n = 3. ^*P < 0.05; ^**P < 0.01